Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; HindIII/XbaI--3.0, 2.1; PstI--4.0, 0.9, 0.3; BamHI--3.4, 1.7. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.07, 0.99, 1.26; BglI--0.31; SacI--0.87; BamHI--1.69. |