| 產(chǎn)品名稱(chēng) |
pLDLR-2 |
| 商品貨號(hào) |
B227662 |
| Designations |
pLDLR-2 |
| Species |
Homo sapiens, human |
| Depositors |
DW Russell |
| Applications |
in another host, produces protein low density lipoprotein receptor (familial hypercholesterolemia) low density lipoprotein receptor |
| Vector |
Construct size (kb): 8.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: LDLR
Genomic copy number: unique
Gene name: low density lipoprotein receptor (familial
hypercholesterolemia)
Contains complete coding sequence?: U
Chromosome: 19; Localization: 19 p13.2
Tissue: fetal adrenal gland
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 5.3
Cross references: Insert lengths(kb): 5.300000190734863 Tissue: fetal adrenal gland Gene product: low density lipoprotein receptor (familial hypercholesterolemia)(low density lipoprotein receptor) [LDLR] Alleles: A2, E1, E2, A1, A2, B1, B2, D1, D2, K1, K2, Q1, Q2, S2, A1, S1 Target Gene: low density lipoprotein receptor (familial hypercholesterolemia) |
| Insert Size (kb) |
5.300 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--3.0, 1.8, 1.4, 1.1, 0.8; xbaI--uncut; BamHI--3.0, 1.9, 1.7, 1.4, 0.32; HindIII--8.0; EcoRI--8.0. Encodes nt 1-2580 of the published sequence, including the entire coding region and 3' untranslated region and a portion of the 5' untranslated region. When pLDLR-2 is transfected into COS-M6 cells, protein is expressed that binds low-density lipoprotein and that is recognized by monoclonal antibody IgG-C7, specific to the human receptor. The vector contains a portion of pBR322 and SV40 sequences including SV40 ori, 16S and 19S donor and acceptor splicing sites, and polyadenylation signals. Constructed by joining the cDNA insert of p203 (nt 1-323) and p101 (nt 324-5144) via overlapping HgiAI sites. |
| References |
Yamamoto T, et al. The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell 39: 27-38, 1984. PubMed: 6091915
Brown MS, et al. Methods and compositions for the detection of familial hypercholesterolemia. US Patent 4,966,837 dated Oct 30 1990
Sudhof TC, et al. The LDL receptor gene: a mosaic of exons shared with different proteins. Science 228: 815-822, 1985. PubMed: 2988123
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
