Comments |
Restriction digests of the clone give the following sizes (kb): BglII--12.0; HindIII--9.8, 1.7; PstI--4.6, 3.7, 3.2; SalI--12.0; XhoI--4.6, 4.1, 2.2, 0.8; PvuII--3.6, 2.5, 2.2, 1.8; EcoRI/HindIII--7.1, 2.7, 1.7. The insert contains the following restriction sites (kb from the 5' end): PstI--0.4, 5.0; BglII--2.2; XhoI--4.1, 6.3, 7.1; HindIII--7.2. Contains the complete gene, including transcription initiation and polyadenylation signals. Contains one Alu sequence and an associated microsatellite of the type (GA)n in intron 2 located 2.8 kb from the 5' end. |
References |
Hofmann SL, et al. cDNA and genomic cloning of HRC, a human sarcoplasmic reticulum protein, and localization of the gene to human chromosome 19 and mouse chromosome 7. Genomics 9: 656-669, 1991. PubMed: 2037293
Sandra L Hofmann, personal communication
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