Restriction digests of the clone give the following sizes (kb): BamHI--4.4; XbaI--4.4; PstI--4.4; HindIII--4.4. This is an Escherichia coli/Saccharomyces integrative shuttle vector with multiple cloning sites. Constructed from YEp351 (ATCC 37672) by deleting all 2 micron circle sequences by digestion with HpaI and AatII (nt 2259-3492). |