contains easily purifiable cassette(s) for construction
Comments
Contains promoterless CAT gene flanked by HindIII sites which can be used to construct other vectors.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Close TJ, Rodriguez RL. Construction and characterization of the chloramphnicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements. Gene 20: 305-316, 1982. PubMed: 6299895