Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.3, 17.5, 7.6; EcoRI/HindIII--26.5, 6.2, 3.6, 3.2, 3.1, 1.7, 1.5. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): AAGAGCTTCACGCAGAGCTCGCACC and TGCACCAGGTTGGAGCTCCAGCTGAA. The predicted product size is (bp): 116. The observed product size is (bp): 116. |