Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.1; HindIII--5.0, 2.5; PstI--3.7, 3.8; BamHI--4.4, 3.1; EcoRV--6.4, 0.7, 0.5. The insert contains the following restriction sites (nt from the 5' end): PvuII--332, 441, 1974, 2557; BamHI--424; HindIII--530; EcoRV--2151, 2613. |