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pcDV1
pcDV1
規(guī)格:
貨期:
編號:B230106
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pcDV1
商品貨號 B230106
Designations pcDV1
Depositors H Okayama
Biosafety Level 1
Vector Information
Size (kb): 3.0000000000000000
Vector: pcDV1 (plasmid)
Promoters: Promoter none
Construction: pBR322, SV40
Marker(s):ampR
Construct size (kb): 3.0
Features: marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
enhancer: none
Applications
expression vector
vector for constructing cDNA libraries
Comments
pcDV1 (ATCC 37368) and pL1 (ATCC 37369) should be used together to construct a cDNA library.
The SV40 segment contains KpnI and EcoRI sites for the preparation of vector-primer containing a poly(dT) tail at one end and the polyadenylation sequence from the late region of the virus.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662

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