產(chǎn)品名稱 |
pcDV1 |
商品貨號 |
B230106 |
Designations |
pcDV1 |
Depositors |
H Okayama |
Biosafety Level |
1 |
Vector Information |
Size (kb): 3.0000000000000000 Vector: pcDV1 (plasmid) Promoters: Promoter none Construction: pBR322, SV40 Marker(s):ampR Construct size (kb): 3.0 Features: marker(s): ampR promoter: none replicon: pMB1 terminator: SV40 late enhancer: none |
Applications |
expression vector vector for constructing cDNA libraries |
Comments |
pcDV1 (ATCC 37368) and pL1 (ATCC 37369) should be used together to construct a cDNA library. The SV40 segment contains KpnI and EcoRI sites for the preparation of vector-primer containing a poly(dT) tail at one end and the polyadenylation sequence from the late region of the virus. |
Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
Growth Conditions |
Temperature: 37.0°C |
References |
Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662
|
Related Products |
component of:ATCC 77361 |
Shipping Information |
Distributed: freeze-dried |