HA produced in goose RBC at pH 6.0 to pH 7.0 at 4, 22, and 37°C with optimal temperature of 22°C.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Storage Conditions
?-70°C or colder
Comments
Sensitive to sodium deoxycholate. HA produced in goose RBC at pH 6.0 to pH 7.0 at 4, 22, and 37°C with optimal temperature of 22°C.
Officially recognized as prototype strain by American Committee on Arthropod-borne Viruses.
Effect on Host
In vivo: Death of host animal
Death
Recommended Host
Suckling mouse
Production Host: ?Suckling mouse
Growth Conditions
Recommendations for Infection: Inoculate intracerebrally into suckling mice. Resuspend 10% sMb with 7.5% BSA in PBS.
Incubation: 2-3 days
Name of Depositor
RE Shope
Special Collection
ATCC
Chain of Custody
Deposited as Melao virus
Source
Mosquito pool, Aedes (Ochlerotatus) scapularis, Trinidad, 1955
References
Anderson CR, et al. Melao virus, a new agent isolated from Trinidadian mosquitoes. Am. J. Trop. Med. Hyg. 11: 687-690, 1962. PubMed: 13990007