| 產品名稱 | CHLA-01-MED |
|---|---|
| 商品貨號 | B231873 |
| Organism | Homo sapiens, human |
| Tissue | brain/brain tumor |
| Cell Type | Medulloblastoma |
| Product Format | frozen |
| Morphology | rounded |
| Culture Properties | single cells and tight clusters in suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | medulloblastoma |
| Age | 8 years |
| Gender | male |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | This is a human male cell line with a chromosome range of 42∼46. The karyotype contained two consistent chromosome rearrangements, der(17)t(1;17)(q11;q25) and der(18)(17;18)(q11.2;p11.2) and approximately 40% of the examined metaphase spreads contained 11∼16 double minutes. |
| Images |  |
| Derivation | CHLA-01-MED was established from an 8 year old boy with CNS-disseminated medulloblastoma. Cells were isolated from the brain tumor resected at the time of diagnosis and were cultured in neurobasal medium after mechanical disruption. This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes. When placed in medium containing fetal bovine serum the cells attach and spread, but after a period of proliferation they senesce. A metastatic recurrent medulloblastoma cell line from this patient is available as CHLA-01R-MED (see ATCC CRL-3034) |
| Clinical Data | CHLA-01-MED was established from an 8 year old boy with CNS-disseminated medulloblastoma. male |
| Comments | CHLA-01-MED was established from an 8 year old boy with CNS-disseminated medulloblastoma. Cells were isolated from the brain tumor resected at the time of diagnosis and were cultured in neurobasal medium after mechanical disruption. This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes. When placed in medium containing fetal bovine serum the cells attach and spread, but after a period of proliferation they senesce. The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Children's Cancer to Childrens Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community. A metastatic recurrent medulloblastoma cell line from this patient is available as CHLA-01R-MED (see ATCC CRL-3034) |
| Complete Growth Medium | DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
|
| Subculturing | Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, cultures can be established by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium.
Note: Due to large, tight clusters formed by these cells, it may be difficult to accurately measure cell number and viability.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended. Medium renewal: Add fresh medium every 4 to 7 days (depending on cell density). |
| Cryopreservation | Freeze medium: fetal bovine serum, 50%; Cell Freezing Medium-DMSO (Sigma, Cat. No. C-6295), 50% |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X Y CSF1PO: 11 D13S317: 8, 14 D16S539: 9, 11 D5S818: 11, 13 D7S820: 10 THO1: 6, 9.3 TPOX: 9, 12 vWA: 16, 17 |
| Name of Depositor | Anat Erdreich-Epstein, Saban Research Institute, Childrens Hospital Los Angeles |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
