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pMCT15 [RW-139]
pMCT15 [RW-139]
規(guī)格:
貨期:
編號(hào):B232294
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pMCT15 [RW-139]
商品貨號(hào) B232294
Designations pMCT15 [RW-139]
Species Homo sapiens, human
Applications
Subcloned from cMCT15 isolated by YN-12 oligonucleotide; RFLPs observed under normal stringency (0.1X SSC).
Vector
Construct size (kb): 5.5
Insert
DNA: genomic
Insert lengths(kb): 2.700000047683716
Gene product: DNA Segment, single copy [D21S113]
Insert Size (kb) 2.700
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): TaqI--0.75, 0.5 + smaller; BamHI--5.5; EcoRI--5.5; HindIII--5.5; PvuII--2.3, 1.55, 0.6, 0.55, 0.4.
The MspI polymorphism demonstrates codominant segregation in 40 three generation families.
Subcloned from cMCT15 isolated by YN-12 oligonucleotide; RFLPs observed under normal stringency (0.1X SSC).
Enzyme(s) not detecting polymorphism: RsaI, HindIII.
References

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pMCT15) on chromosome 21 [D21S113]. Nucleic Acids Res. 16: 9882, 1988. PubMed: 2903484

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

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