Vector Information |
Size (kb): 4.2740001678466800 Vector: pRS404 (phagemid) Promoters: Promoter T7 Construction: pBluescript II KS+ Marker(s):TRP1,ampR Construct size (kb): 4.27400016784668 Features: insert detection: lacZ' marker(s): TRP1 marker(s): ampR promoter: T3 promoter: T7 promoter: lac replicon: f1 replicon: pMB1 MCS: KpnI...SacI |
Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--3.35, 0.95; BglI--2.6, 1.7; PvuII--3.85, 0.45. A YI-type shuttle vector with TRP1 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites. |