產(chǎn)品名稱(chēng) |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
商品貨號(hào) |
B232473 |
Deposited As |
Saccharomyces cerevisiae Hansen, teleomorph |
Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
Strain Designations |
MW3271-10C pms1-delta |
Alternate State |
Candida robusta Diddens et Lodder |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen |
Type Strain |
no |
Genotype |
MATa pms1-delta leu2-3 leu2-112 lys2-20 his4-519 ade8-deltaKpn ura3-52 hom3-10 thr1 lys1-1 ade2 arg4-deltaSst1
RefWilliamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926, RefKramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974, (Isogenic with MW3271-10C (ATCC 208348) except for the pms locus.) |
Preceptrol® |
no |
Mating Type |
a |
Ploidy |
haploid |
Comments |
Meiosis-sporulation: postmeiotic segregation increase (CL2) |
Medium |
ATCC® Medium 1069: YPAD medium
|
Growth Conditions |
Temperature: 25.0°C Atmosphere: Typical aerobic Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation. |
Subcultivation |
Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation. |
Name of Depositor |
YGSC |
Special Collection |
Yeast Genetic Stock Center |
Chain of Custody |
ATCC <<--YGSC<<--W. Kramer and K. Lusnak |
References |
Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926
Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974
Isogenic with MW3271-10C (ATCC 208348) except for the pms locus.
Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926
Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974
Isogenic with MW3271-10C (ATCC 208348) except for the pms locus.
|