Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4, 12.0; EcoRI--16.4; HindIII--16.4. Codominant segregation demonstrated in 53 3-generation families. MspI also detects a 2-allele polymorphism which has not yet been verified by family studies. Enzyme(s) not detecting polymorphism: RsaI, BglII, PstI, PvuII. |