Restriction digests of the clone give the following sizes (kb): EcoRI--4.9; BamHI--4.9; EcoRI/BamHI--4.9; HindIII--4.9; PstI--2.9, 1.4; EcoRI/HindIII--2.7, 2.3. Codominant segregation demonstrated in 39 3-generation families. RFLPs observed under normal conditions of hybridization and washing. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, PstI, PvuII, RsaI, TaqI. |