Genetics Institute, Inc., A Lin, Genetics Institute, Inc.
Applications
in another host, produces protein calcium-dependent phospholipid-binding protein
Vector
Construct size (kb): 7.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.845999956130981
Tissue: histiocytic lymphoma U-937 cell line
Gene product: calcium-dependent phospholipid-binding protein [PLA2*]
Target Gene: calcium-dependent phospholipid-binding protein
Insert Size (kb)
2.846
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: DNA supplied by depositor (dried) (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.1, 0.76; NcoI--3.2, 1.6, 1.5, 1.4; SalI--4.6, 2.8.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.01, 0.36; EcoRI--0.77; EcoRV--1.00; NcoI--0.73, 2.26; PvuII--1.69; XbaI--0.52.
References
Clark JD, et al. A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP. Cell 65: 1043-1051, 1991. PubMed: 1904318
Knopf JL, Clark J. DNA sequences encoding phospholipase A2 enzyme and processes for producing enzyme. US Patent 5,322,776 dated Jun 21 1994
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.