Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.6 (doublet), 5.2, 0.76; EcoRI/HindIII--22.6, 13.5, 6.6, 5.0, 0.88, 0.76, 0.38. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): GGATCTCTCACTGAAGCCTCG and CACCTGATTCAGCATCAGACAAT. The predicted product size is (bp): 252. The observed product size is (bp): 250. |