Vector |
DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: XhoI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ', <- primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, <- promoter: lac, <- primer site: M13 reverse, <- replicon: pMB1 marker(s): ampR, <- Cross references: |
Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: rat Gene name: DNA Segment, expressed Contains complete coding sequence?: U Strain: Sprague-Dawley Tissue: incisor, maxillary and mandibular (apical portion and entire pulp) from animals 3-4 weeks old Type of DNA: cDNA Insert 5' end: EcoRI Modification: EcoRI adaptor Insert 3' end: XhoI Modification: XhoI primer Insert size (kb): Cross references: DNA Seq. Acc.: R47182 Tissue: incisor, maxillary and mandibular (apical portion and entire pulp) from animals 3-4 weeks old Gene product: DNA Segment, expressed |