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pCN51
pCN51
規(guī)格:
貨期:
編號:B234129
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCN51
商品貨號 B234129
Designations pCN51
Depositors R Diaz Orejas
Biosafety Level 1
Vector Information
Size (kb): 5.9000000953674320
Vector: pCN51 (plasmid)
Construction: pCN50
Marker(s):kanR
Construct size (kb): 5.900000095367432
Features: marker(s): kanR
replicon: repA, pMB1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--4.4, 1.4; EcoRI--4.8, 1.0; PvuII--5.8.
Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences.
Retains the mobilization site of pBR322-derived vectors and can be mobilized if suitable functions are provided in trans.
Shuttle vector.
A spontaneous deletion derivative of pCN50 (77099).
The order of the major features in this plasmid is: PvuII - pMB1 ori - BamHI - kanR - HindIII - repA.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095

Ramon Diaz Orejas, personal communication

Shipped frozen
Shipping Information Frozen glycerol stock of ?E. coli containing the plasmid.
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胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
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