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pPC1A
pPC1A
規(guī)格:
貨期:
編號(hào):B234150
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pPC1A
商品貨號(hào) B234150
Designations pPC1A
Species Escherichia coli (Migula) Castellani and Chalmers
Depositors IK Yu
Applications
in another host, produces protein
Insert
DNA: cDNA
Insert lengths(kb): 2.799999952316284
Gene product: [pPC1A]
Insert Size (kb) 2.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen
Comments
A gene encoding an endocellulase from Clostridium strain IY-2 which can digest bamboo fibers, cellulose, rice straw, and sawdust, was isolated by shotgun cloning in an E.coli expression plasmid pLC2833.
E.coli k-12 comprising a vector, pLC2833 which contains a promoter and a 2.8 kb Clostridium strain IY-2 DNA insert which is further cleaved in to 2.2 kb DNA fragment comprising at least an entire structural gene and signal sequences of cellulase.
Classification Enterobacteriaceae, Escherichia
References

Remaut E, et al. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene 15: 81-93, 1981. PubMed: 6271633

Yu IK. Secretion of Clostridium cellulase by E. coli. US Patent 5,496,725 dated Mar 5 1996

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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