Comments |
Restriction digests of the clone give the following sizes (kb): NotI-- > 9.0; EcoRI-- >9.0, 0.7, 0.6; SacI-- >9.0, 7.0, 2.3 (doublet), 1.3, 0.85, 0.7; XbaI-- >9.0, 8.0, 2.7, 1.2, 1.1, 1.0, 0.5. The insert contains the following restriction sites (approximate kb from 5' end): EcoRI--11.70, 12.20, 12.70; SacI--6.95, 9.60, 12.00; XbaI--2.65, 2.75, 3.75, 3.85, 4.35, 5.45, 6.35. ATCC 99549 contains exons 4-7 of the 14 exons which comprise the norepinephrine transporter gene. |