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pcD(SRalpha)-F115 [moIL-10 cDNA]
pcD(SRalpha)-F115 [moIL-10 cDNA]
規(guī)格:
貨期:
編號:B234275
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pcD(SRalpha)-F115 [moIL-10 cDNA]
商品貨號 B234275
Designations pcD(SRalpha)-F115 [moIL-10 cDNA]
GenBank Number

M37897

Species Mus musculus, mouse
Depositors DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc.
Applications
in another host, produces protein interleukin 10
Vector
Construct size (kb): 4.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.340999960899353
Tissue: T-lymphocyte D10 cell line, conconavalin A-treated
Gene product: interleukin 10 [Il10]
Target Gene: interleukin 10
Insert Size (kb) 1.341
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.4, 1.5; EcoRI--4.9; PstI--2.1, 1.55, 0.65, 0.4, <0.3; HindIII--3.1, 1.8; SstI--3.9, 0.6, 0.4.
Contains the complete coding sequence (nt 76-612).
The fragment excised by BamHI consists of the insert plus some vector sequence.
The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR).
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Mosmann TR, et al. Nucleic acids encoding cytokine synthesis inhibitory factor (interleukin-10). US Patent 5,231,012 dated Jul 27 1993

Moore KW, et al. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI. Science 248: 1230-1234, 1990. PubMed: 2161559

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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