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HFBCI15
HFBCI15
規(guī)格:
貨期:
編號:B234561
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HFBCI15
商品貨號 B234561
Designations HFBCI15
GenBank Number

M85435

Species Homo sapiens, human
Vector
Construct size (kb): 5.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CYC1
Genomic copy number: unique
Gene name: cytochrome c-1
Contains complete coding sequence?: U
Chromosome: 8; Localization: 8 q24.3
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.35
Cross references: DNA Seq. Acc.: M85435; EST: EST01951; Primer: M13 Forward;
THC id: THC123464
Insert lengths(kb): 2.349999904632568
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: cytochrome c-1(cytochrome c1) [CYC1]
Insert Size (kb) 2.350
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.45, 0.6, 0.3; BamHI--4.5, 0.8; PstI--4.5, 0.8.
EST01951 matches GenBank accession HUMCYCAA with 95.95% identity over the sequence range: 73-245.
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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