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cDMD 2b-3 [30-2, cDMD2b-3]
cDMD 2b-3 [30-2, cDMD2b-3]
規(guī)格:
貨期:
編號(hào):B234920
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 cDMD 2b-3 [30-2, cDMD2b-3]
商品貨號(hào) B234920
Designations cDMD 2b-3 [30-2, cDMD2b-3]
GenBank Number

M18533

Species Homo sapiens, human
Applications
For plasmid amplification, use a lac Iq host.
Vector
Construct size (kb): 4.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 1.149999976158142
Tissue: fetal muscle
Gene product: dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272 [DMD]
Alleles: U2, A1, A2, C1, T1, U1, A1, A1, A2, A2, B1, B2, C2, D2, R1, R2, T1, T2, T2, T3, D1
Insert Size (kb) 1.150
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--2.8, 1.2; BglI--2.5, 1.5; XbaI--4.3.
For plasmid amplification, use a lac Iq host.
Inserted into EcoRI site with 5' end closer to T7 promoter. Contains internal BglII (2) and XbaI sites.
ATCC 57668 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not needed for ATCC 57668, but is for ATCC 57669.
Includes probes 2b and 3. Detects genomic HindIII fragments of 10.5, 4.2, 6.6, 2.7, 6, 1.7, 12, 3, and 7.3 kb.
Of the DMD cDNA clones, 1-2a includes nucleotides 1-1538; 2b-3: 1455 to approximately 2600; 4-5a: 2600-4550; 5b-7: 4400-6900; 8: 6900-7800; 9-14: 7800-13900 (in base pairs from the 5' end of the cDNA).
References

Lindlof M, et al. Gene deletions in X-linked muscular dystrophy. Am. J. Hum. Genet. 44: 496-503, 1989. PubMed: 2929594

Den Dunnen JT, et al. Topography of the Duchenne muscular dystrophy (DMD) gene: FIGE and cDNA analysis of 194 cases reveals 115 deletions and 13 duplications. Am. J. Hum. Genet. 45: 835-847, 1989. PubMed: 2573997

Lucotte G, et al. Molecular deletion patterns in Duchenne muscular dystrophy patients. Ann. Genet. 32: 214-219, 1989. PubMed: 2610487

Dominguez-Steglich M, et al. The dystrophin gene is autosomally located on a microchromosome in chicken. Genomics 8: 536-540, 1990. PubMed: 2286374

Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877

Koenig M, et al. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein. Cell 53: 219-228, 1988. PubMed: 3282674

Louis M Kunkel, personal communication

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