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JH-5
JH-5
規(guī)格:
貨期:
編號(hào):B235283
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 JH-5
商品貨號(hào) B235283
Designations JH-5
Species Homo sapiens, human
Depositors H Donis-Keller, JR Howe
Vector
Construct size (kb): 44.90000152587891
Insert
DNA: genomic
Insert lengths(kb): 15.69999980926514
Gene product: DNA Segment, single copy [D12S54]
Alleles: A1, B1, B2, C1, C2, A2
Insert Size (kb) 15.700
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--20.0, 9.2, 7.0, 6.6, 0.8, 0.7; XbaI--23.0, 8.2, 5.6, 3.8, 3.2, 1.7; BamHI/SalI--20.0, 9.2, 8.6, 6.6; BglII--22.0, 14.0, 7.4, 0.7; Uncut; SalI--20.0, 9.2, 15.7.
Restriction digests of the clone give the following sizes (kb): BamHI/SalI--5.2, 4.8, 4.7 (insert), 20.0, 9.2 (vector).
The BglII and TaqI allele systems are in strong linkage disequilibrium. There is an additional variable BglII fragment at 1.8 kb which may be a dominant polymorphism.
Subcloned from YAC D61E6.
Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HIndIII, MspI, PstI (in a panel of 6 individuals).
References

Howe JR, et al. A new RFLP marker D12S54 maps between F8VWF and KRAS2 on human chromosome 12p. Nucleic Acids Res. 19: 2512, 1991. PubMed: 1675000

Helen Donis-Keller, personal communication

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