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YEp358
YEp358
規(guī)格:
貨期:
編號:B235319
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 YEp358
商品貨號 B235319
Designations YEp358
Depositors CJ Lusty
Biosafety Level 1
Vector Information
Size (kb): 8.0000000000000000
Vector: YEp358 (plasmid)
Promoters: Promoter none
Construction: YEp355 (ATCC 37727), pUC18
Marker(s):URA3,ampR
Construct size (kb): 8.0
Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
promoter-cloning vector
shuttle vector
Comments
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-3; SacI-1; KpnI-1; SmaI-3; BamHI-3; XbaI-3; SalI-3; PstI-1; SphI-1; HindIII-3. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): HindIII--8.0; EcoRI--8.0.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37731 - 37733) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'G AAT TCG AGC TCG GTA CCC GGG GAT CCT CTA GAG TCG ACC TGC AGG CAT GCA AGC TTC GAT CCC 3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

Shipped freeze-dried
Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid.

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