產(chǎn)品名稱 |
pGIKS-BioD |
商品貨號(hào) |
B235360 |
Designations |
pGIKS-BioD |
GenBank Number |
J04423
5597)">J04423 (4947->5597)
|
Species |
Escherichia coli (Migula) Castellani and Chalmers |
Depositors |
M Follettie |
Applications |
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). |
Vector |
Construct size (kb): 3.56 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS+ Intact vector size: 2.961 Type of vector: phagemid Vector end: KpnI Vector end: SacI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ' marker(s): ampR replicon: pMB1, f1 Cross references: Vector end (5'): KpnI Vector end (3'): SacI Excise insert: XhoI+SmaI Promoter giving the sense strand: T3 Promoter giving the antisense strand: T7 |
Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Escherichia coli Gene symbol: bioD Gene name: dethiobiotin synthetase Contains complete coding sequence?: N Type of DNA: genomic Insert 5' end: XhoI Modification: KpnI linker Insert 3' end: SmaI Modification: SacI linker Insert size (kb): 0.66 Cross references: DNA Seq. Acc.: J04423 Nucleotides 1-651 of the insert correspond to nucleotides 4947-5597 of J04423. Insert lengths(kb): 0.66 Gene product: dethiobiotin synthetase [bioD] Insert end (5'): KpnI linker Insert end (3'): SacI linker |
Insert Size (kb) |
0.660 |
Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information |
Distributed: freeze-dried |
Comments |
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert contains nt 4947-5597 of Genbank J04423. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.18; PvuII--0.18; RsaI--0.64. The following restriction enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI. Restriction digests of the clone give the following sizes (kb): SmaI--3.6; SmaI/XhoI--3.0, 0.6; XhoI--3.6. |
Classification |
Enterobacteriaceae, Escherichia |