Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Preceptrol®
no
Genotype
Toxinotype: IIIc;
Type Strain
no
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
RefLemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Confirmed by CDC: NAP1, Toxinotype III, binary toxin positive
Binary Toxin gene cdtB was amplified by PCR
Toxinotype IIIc
Ribotype 027
Medium
ATCC® Medium 2107: Modified Reinforced Clostridial
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor
R Clark
References
Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Stubbs, S et al. Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile. FEMS Microbiol. Lett. 186(2): 307-312, 2000. PubMed: 10802189
Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986