国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pAMS
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pAMS
pAMS
規(guī)格:
貨期:
編號:B235785
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pAMS
商品貨號 B235785
Designations pAMS
GenBank Number

AF010170

Species Murine leukemia virus
Depositors CA Wilson, D Miller
Applications
AM-MLV (ATCC VR-1448) is an engineered broad host range helper virus.
This AM-MLV clone can be used as a reference standard for detection of murine retroviruses.
Vector
Construct size (kb): 11.32800006866455
Insert
DNA: genomic
Insert lengths(kb): 9.03600025177002
Gene product: [AM-MLV*]
Insert Size (kb) 9.036
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Frozen glycerol stock of ?E. coli containing the plasmid

Comments
Restriction digests of the clone give the following sizes (kb): XhoI--6.8, 4,8; SalI--12.0; BamHI--7.8, 3.6.
The plasmid contains the following restriction sites (approximate kb from nt 1 of the GenBank record): BamHI--3.82, 4.13, 7.55; BglI--4.51, 5.98, 7.30, 10.46; ClaI--5.57, 8.23; EcoRI--0.00, 6.96; HindIII--5.49, 5.65, 7.45; KpnI--0.63, 3.45, 8.86;
NdeI--5.99, 9.27; PstI--1.16, 1.33, 10.58; PvuI--1.01, 2.71, 10.71; SacI--0.56, 3.15, 8.79; SalI--4.30; SmaI--0.62, 5.10, 8.85; XbaI--0.44, 5.92, 8.67; XhoI--2.15, 6.92.
AM-MLV (ATCC VR-1448) is an engineered broad host range helper virus. It is a fusion of amphotropic virus 4070A pol-env with Mo-MLV gag region. It can infect a wide range of mouse cell types (including BALB/c) as well as human and chicken cells.
This AM-MLV clone can be used as a reference standard for detection of murine retroviruses.
The Hybrid Amphotropic/Moloney Murine Leukemia Virus, pAMS, was constructed by inserting the 1541bp SalI-ClaI 4070A pol-env containing fragment into homologous region of Mo-MLV using the cloning vector pBR322 as a backbone.
Classification Retroviridae, Gammaretrovirus, Mammalian virus group
References

Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479