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Size (kb): 3.9119999408721920

Vector: pHisGal (phagemid)

Promoters: Promoter for expression lac

Construction: pQE9, Gal4 DNA-binding domain

Marker(s):ampR

Construct size (kb): 3.911999940872192

Features: marker(s): ampR
operator: Gal4 DNA-binding domain (aa 1-147)
promoter for expression: lac
replicon: pMB1
MCS: SmaI...HindIII
restriction site: BamHI
coding sequence: promoterless cat gene
epitope tag: hexahistidine sequence (6-His)

expression vector

vector permitting construction of fusion proteins

Restriction digests of the clone give the following sizes (kb): HindIII--4.0; PvuI--4.0; XbaI--4.0.

Plasmid contains the following restriction sites (approximate kb from the BamHI site): XbaI--1.51; PvuI--3.15.

Expression vector for production of Gal4 fusion proteins, allowing protein purification by affinity chromatography through a nickel nitrilotriacetic acid (Ni-NTA) column.

For expression of cloned inserts, the medium should contain 10 uM zinc chloride and expression can be induced using IPTG.

Temperature: 37.0°C