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pKLAf1IIRM520-4
pKLAf1IIRM520-4
規(guī)格:
貨期:
編號:B238513
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pKLAf1IIRM520-4
商品貨號 B238513
Designations pKLAf1IIRM520-4
Species Anabaena flos-aquae (Lyngbye) Brebisson, blue-green algae
Depositors New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Vector
Construct size (kb): 16.20
Insert
DNA: genomic
Insert lengths(kb): 12.4
Gene product: modification methylase AflII
Gene product: restriction endonuclease AflII
Insert Size (kb) 12.400
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--11.5, 3.4, 2.3; BamHI--17.0; HindIII--7.8, 4.4, 2.5, 1.1, 0.4; PstI--12.0, 4.7.
The insert contains the following restriction sites (approximate kb from the 5' XhoII site): EcoRI--2.8, 5.0; HindIII--8.1, 10.6, 11.05, 12.05; NsiI--8.0; PstI--1.8; EcoRV--5.2; NcoI--11.8; SpeI--2.9, 5.5, 9.7; XhaII--2.1.
Distributed in aliquots of 250 ng (10 ng/ul).
References

Lunnen KD, Wilson GG. Method for producing the AflII restriction endonuclease and methylase. US Patent 5,030,569 dated Jul 9 1991

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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