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pHPT20
pHPT20
規(guī)格:
貨期:
編號(hào):B238682
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pHPT20
商品貨號(hào) B238682
Designations pHPT20
Species Cricetus cricetus, hamster
Depositors C Caskey
Vector
Construct size (kb): 5.050000190734863
Insert
DNA: cDNA
Insert lengths(kb): 0.75
Tissue: V79 cell line
Gene product: hypoxanthine phosphoribosyltransferase [HPRT]
Insert Size (kb) 0.75
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
The depositor indicates the restriction map is the same as for mutant cDNA pHPT12.
Contains the coding region and a retroviral LTR.
There is a high degree of homology within the protein coding region between the mouse (ATCC 37424) and the hamster (ATCC 37430) HPRT.
References

Konecki DS, et al. Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis od cDNA recombinants. Nucleic Acids Res. 10: 6763-6775, 1982. PubMed: 6294614

Brennand J, et al. Expression of human and Chinese hamster hypoxanthine-guanine phosphoribosyltransferase cDNA recombinants in cultured Lesch-Nyhan and Chinese hamster fibroblasts. J. Biol. Chem. 258: 9593-9596, 1983. PubMed: 6688416

C. Thomas Caskey, personal communication

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