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pKG1800
pKG1800
規(guī)格:
貨期:
編號(hào):B239747
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pKG1800
商品貨號(hào) B239747
Designations pKG1800
Depositors M Rosenberg
Biosafety Level 1
Vector Information
Size (kb): 4.9000000953674320
Vector: pKG1800 (plasmid)
Construction: pKO1
Marker(s):ampR
Construct size (kb): 4.900000095367432
Features: insert detection: galK
marker(s): ampR
replicon: pMB1
Applications
terminator-cloning vector
Comments
Insertion of terminator sequences at the cloning sites causes the galK+ phenotype to become galK-. galK+ plasmids are lethal on galETK hosts because of galactose-1-phosphate accumulation, so terminator-containing clones survive in galactose media.
A terminator cloning plasmid vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

McKenney K, et al. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif. Anal. 2: 383-415, 1981. PubMed: 6101056

Nierman WCVectors for cloning promoters and terminatorsIn: Nierman WCVectors: a survey of molecular cloning vectors and their usesBostonButterworthpp. 153-177, 1988

Shipped freeze-dried
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