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humfact7
humfact7
規(guī)格:
貨期:
編號:B241357
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 humfact7
商品貨號 B241357
Designations humfact7
GenBank Number

J02933

Species Homo sapiens, human
Depositors PJ O'Hara
Vector
Construct size (kb): 5.099999904632568
Insert
DNA: cDNA
Insert lengths(kb): 2.400000095367432
Tissue: hepatocellular carcinoma Hep G2 cell line
Gene product: coagulation factor VII (serum prothrombin conversion accelerator) [F7]
Insert Size (kb) 2.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
The following enzymes cut the insert once (nt from 5' end, short leader): BssHII-139 MluI-147 XmaIII-167 BglII-228 ScaI-443 Eco47III-470 XbaI-562 NcoI-635 BclI-687 SstII-714 SplI-840 Asp718-1209 KpnI-1213 ApaLI-1996 NsiI-2246.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.6; SacI--3.5, 1.5, 0.45; EcoRI/SacI/XhoI--2.9, 1.5, 0.7, 0.45; BglI--4.3, 1.2.
The insert includes the entire coding sequence of the gene.
There are two alternate cDNAs for F7, one encoding a leader of 60 amino acids and one a leader of 38 amino acids. This clone encodes the short leader.
The depositor indicates the polymorphism has allele lengths that differ by only a few nucleotides. It would be difficult to detect this polymorphism by means of a Southern blot of the genomic digest.
References

O'Hara PJ, Grant FJ. The human factor VII gene is polymorphic due to variation in repeat copy number in a minisatellite. Gene 66: 147-158, 1988. PubMed: 2970988

Hagen FS, et al. Characterization of a cDNA coding for human factor VII. Proc. Natl. Acad. Sci. USA 83: 2412-2416, 1986. PubMed: 3486420

Huang Q, et al. Substrate recognition by tissue factor-factor VIIa. J. Biol. Chem. 271: 21752-21757, 1996. PubMed: 8702971

O'Hara PJ, et al. Nucleotide sequence of the gene coding for human factor VII, a vitamin K-dependent protein participating in blood coagulation. Proc. Natl. Acad. Sci. USA 84: 5158-5162, 1987. PubMed: 3037537

Patrick J O'Hara, personal communication

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