產(chǎn)品名稱 |
Haemophilus influenzae |
商品貨號(hào) |
B81021 |
中文名稱 |
流感嗜血桿菌 |
其它保藏中心編號(hào) |
ATCC10211 |
特征特性 |
1. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. 2. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8. 3. Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. 4. API NH. bioMerieux. 5. Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A. 6. Medical microbiology -- Culture media -- Part 2: Ready-to-use blood culture systems. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-2: 2004, 2004 |
具體用途 |
ATCC10211是標(biāo)準(zhǔn)流感嗜血桿菌菌株,但不是模式菌株。ATCC10211用于 培養(yǎng)基測(cè)試; 藥敏試驗(yàn); 紙片擴(kuò)散法藥敏試驗(yàn); 呼吸道疾病的研究; 質(zhì)控標(biāo)準(zhǔn)菌株。 |
培養(yǎng)基 |
BHI肉湯 BHI 瓊脂 GC肉湯 GC瓊脂 |
培養(yǎng)溫度 |
37 ℃, 5% CO2 |
凍干物說(shuō)明 |
·凍干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培養(yǎng)液或者無(wú)菌水溶解,接種在1-2個(gè)平板上,因凍干菌種處于休眠狀態(tài),請(qǐng)勿接種多個(gè)平板,以免因接種量不足而導(dǎo)致復(fù)蘇不成功; |
保存說(shuō)明 |
1、長(zhǎng)期保存的儲(chǔ)存菌株以冷凍干燥保藏為主,特殊要求的菌種可用其它方法進(jìn)行保存,保存期可長(zhǎng)達(dá)幾年至幾十年。2、半儲(chǔ)存菌株以含20%甘油的肉湯或液體石臘封存的半固體瓊脂為主,在0℃以下保存,保存期為3-6個(gè)月。3、應(yīng)用菌株以瓊脂斜面為主,在4℃保存,保存期為1-3個(gè)月。4、菌種的傳代不可超過(guò)6次。視菌種的特性及使用情況,一般儲(chǔ)存菌株每5年傳記代一次,每1-2年從儲(chǔ)存菌株轉(zhuǎn)接至半儲(chǔ)存菌株,半儲(chǔ)存菌株每3-6個(gè)月傳代一次,每1-3個(gè)月從半儲(chǔ)存菌株轉(zhuǎn)接接至應(yīng)用菌株。 |